EVALUATION OF TOXICITY OF 0.2% CHLORHEXIDINE GLUCONATE AND 0.15% BENZYDAMINE HCL (COMBINED) WITH 0.2% CHLORHEXIDINE GLUCONATE (ALONE) ON ORAL CELL LINE

Aim:The purpose of this in vitro study wasto evaluate whether the toxic effects are reduced by using combination 0.5% Benzydamine hydrochloride along with 0.2% Chlorhexidine gluconate and to compare it 0.2 % alone. Materials Methods: Two groups a total 160 cultured cell samples consisting 80 each Control group (0.2% Gluconate alone)and Experimental (Combination 0.15% Hydrochloride). Again subdivided as A Bwith 40 each, viable count dye exclusion test proliferation rate MTT assay, for 30 seconds 60 seconds. Result: Mean andMean ratewas found be higher experimental groupas compared control when exposed secondsas (p-value<0.001). Conclusion: The results indicated that Gluconate, used alone, is more cytotoxic whereas same concentration Hydrochloride less cells thus maintaining their viability. Clinical Significance:This adds value use chlorhexidine regimen over therapy achieve periodontal health.